Calcium ions trigger the exposure of phosphatidylserine on the surface of necrotic cells
Fig 5
Mutations in crt-1 inhibit the exposure of PS on the surface of necrotic cells in deg-1 and unc-8 dominant mutant animals.
Newly hatched L1 larvae of labeled genotypes expressing the PS reporter Pced-1mfg-e8::mKate2 or Pced-1mfg-e8::mCherry are scored for the the number of necrotic cells (A and D) and the relative PS intensity (C and F). All strains carry the deg-1(u38ts) allele were incubated at 25°C. (A and D) The mean numbers of necrotic cells in the head of each newly hatched larva (A) or in the entire body of a late L1/early L2 stage larva (D) are represented as bars. Error bars represent s.e.m.. Numbers in parentheses indicate the number of animals analyzed for each strain. “**”, <0.001<p<0.01, Student t-test. (B) DIC (a, c, e) and mKate2 (b, d, f) images of necrotic cells (arrowheads) in the heads of L1 larvae. Scale bars are 15μm. (E) DIC (a, c) and mCherry (b, d) images of necrotic cells (arrowheads) in the tails of ealy L2 larvae. Scale bars are 10μm. (C and F) The normalized relative signal intensities of PS on the surfaces of necrotic cells in the heads (C) or tails (F) are presented as the mean ratios relative to crt-1(+) worms. Bars represent the mean values of each strain. Error bars represent s.e.m.. Numbers in parentheses represent the number of necrotic cells analyzed. “***”, p<0.001, Student t-test.