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Activation of cell adhesion and migration is an early event of platelet-rich plasma (PRP)-dependent stimulation of human adipose-derived stem/stromal cells

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Abstract

Stem cell therapy is a promising treatment in regenerative medicine. Human adipose-derived stem/stromal cells (hASCs), a type of mesenchymal stem cell, are easy to harvest. In plastic and aesthetic surgery, hASC may be applied in the treatment of fat grafting, wound healing, and scar remodeling. Platelet-rich plasma (PRP) contains various growth factors, including platelet-derived growth factor (PDGF), which accelerates wound healing. We previously reported that PRP promotes the proliferation of hASC via multiple signaling pathways, and we evaluated the effect of PRP on the stimulation of hASC adhesion and migration, leading to the proliferation of these cells. When hASCs were treated with PRP, AKT, ERK1/2, paxillin and RhoA were rapidly activated. PRP treatment led to the formation of F-actin stress fibers. Strong signals for integrin β1, paxillin and RhoA at the cell periphery of RPR-treated cells indicated focal adhesion. PRP promoted cell adhesion and movement of hASC, compared with the control group. Imatinib, an inhibitor of the PDGF receptor tyrosine kinase, inhibited the promotion of PRP-dependent cell migration. PDGF treatment of hASCs also stimulated cell adhesion and migration but to a lesser extent than PRP treatment. PRP promoted the adhesion and the migration of hASC, mediated by the activation of AKT in the integrin signaling pathway. PRP treatment was more effective than PDGF treatment in enhancing cell migration. Thus, the ability of PRPs to promote migration of hASC to enhance cell growth is evident.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank Ms. Mie Aoki and members of the Central Research of Laboratory of Kansai Medical University for their technical assistances.

Funding

This study was supported by Grant-in-Aid for Scientific Research (C) No. 22K09898 from the Ministry of Education, Science, Sports, of Japan.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1, Hirakata, Osaka, 573-1010, Japan

    Michika Fukui, Fangyuan Lai, Masakatsu Hihara, Toshihito Mitsui, Yuki Matsuoka, Zhongxin Sun, Sakurako Kunieda, Shigeru Taketani & Natsuko Kakudo

  2. Department of Microbiology, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan

    Tokifumi Odaka & Kazu Okuma

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  1. Michika Fukui
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Correspondence to Michika Fukui.

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The study was approved by the Ethics Review Board of Kansai Medical University in accordance under the ethical guidelines of the Helsinki Declaration of 1975. All specimens were collected and used with informed consent from the donors. Informed consent was written. The date range of recruitment was from April 2015 to March 2019.

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Fukui, M., Lai, F., Hihara, M. et al. Activation of cell adhesion and migration is an early event of platelet-rich plasma (PRP)-dependent stimulation of human adipose-derived stem/stromal cells. Human Cell 37, 181–192 (2024). https://doi.org/10.1007/s13577-023-00989-1

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